Measuring Metabolic Rates: A Manual for ScientistsOxford University Press, 14.05.2008 - 216 Seiten Measuring metabolic rates is central to important questions in many areas of scientific research. Unfortunately these measurements are anything but straightforward, and numerous pitfalls await the novice and even the experienced investigator. Measuring Metabolic Rates de-mystifies the field, explaining every common variation of metabolic rate measurement, from century-old manometric methods through ingenious syringe-based techniques, direct calorimetry, aquatic respirometry, stable-isotope metabolic measurement and every type of flow-through respirometry. Each variation is described in enough detail to allow it to be applied in practice. Background information on different analyzer and equipment types allows users to choose the best instruments for their application. Respirometry equations - normally a topic of terror and confusion to researchers - are derived and described in enough detail to make their selection and use effortless. Vital topics such as manual and automated baselining, implementing multi-animal systems, and the correct analysis and presentation of metabolic data are covered in enough detail to turn a respirometry neophyte into a hardened metabolic warrior, ready to take on the task of publication in peer-reviewed journals. |
Inhalt
3 | |
7 | |
3 Coulometric Respirometry | 18 |
4 Constant Volume Techniques Using Gas Analysis | 23 |
5 Aquatic Oxygen Analysis | 47 |
6 Direct Calorimetry | 63 |
7 Measuring Field Metabolic Rates | 76 |
Overview | 84 |
12 Validating FlowThrough Respirometry | 132 |
13 Respirometric Data Analysis and Presentation | 136 |
14 The Varieties of Gas Analyzers | 147 |
15 The Varieties of Flow Meters | 158 |
16 The Varieties of Activity Detectors | 167 |
17 The Varieties of Scrubbers Tubing and Tubing Connectors | 171 |
Appendix | 183 |
Notes | 189 |
The Equations | 100 |
10 FlowThrough Respirometry Using Incurrent Flow Measurement | 105 |
11 FlowThrough Respirometry Using Excurrent Flow Measurement | 124 |
Bibliography | 193 |
197 | |
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accurate air stream ambient animal animal’s anode barometric pressure body mass calculate calibration calorimeter calorimetry cathode channel chapter Clark electrode CO2 production correction coulometric data acquisition system desiccant differential pressure dilution Drierite drift effect electrolyte equation example excurrent air excurrent flow Feco2 Feh2o Feo2 Fico2 Figure Fio2 flow control valve flow-through respirometry fractional concentration gas analyzers heat incurrent air incurrent flow rate injection kilopascals layer Lighton magnesium perchlorate main flow manometer mass flow control mass flow meter membrane metabolic rate milliliters multiplexer Nafion nitrogen O2 analysis O2 analyzer O2 and CO2 O2 concentration O2 consumption organism output oxygen electrode paramagnetic partial pressure produces pull system pump recording respirometer chamber respirometry system sample scrub CO2 scrubbed scrubber sensitive sensor setup signal subsampling technique temperature tubing typically usually vapor and CO2 Volo2 voltage volume of O2 water vapor analyzer water vapor pressure zero